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To this end, Klebsiella pneumoniae OU7, a robust strain, was confirmed to rapidly exploit urea and phosphite unconventional chemicals as the primary sources of nitrogen N and phosphorus P , and withstand deliberate contamination in the possibly contaminated systems.
Secondly, metabolic engineering, pathogenicity elimination and adaptive laboratory evolution were successively performed, endowing the best strain with an excellent fermentation performance for safe 2,3-BD production.
Finally, In summary, our technique reduces labor and energy costs and simplifies the fermentation process because sterilization does not need to be performed.
Thus, our work will be beneficial for the sustainable synthesis of 2,3-BD. If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center.
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